Journal:
Article Title: The Bcl-2 family member Bfl-1/A1 is strongly repressed in normal and malignant plasma cells but is a potent anti-apoptotic factor for myeloma cells
doi: 10.1111/j.1365-2141.2004.04908.x
Figure Lengend Snippet: A. Bfl-1/A1 expression was determined using Affymetrix HuGeneFL microarrays on 7 purified CD138+ bone marrow plasma cells (BMPC), 16 purified CD138+ multiple myeloma cells (MM), 7 purified CD20−CD38++ polyclonal plasmablastic cells (PPC), 7 purified CD19+ tonsil B cells (TBC), and 7 purified CD19+ peripheral blood B cells (PBB). Arrays were scaled to an average intensity of 1500 and a threshold of 200 was assigned to small values. The colored image is a graphic representation generated using Cluster and Treeview softwares. Red represents an expression greater than the median, green represents an expression less than the median, and the deeper color intensity represents a greater magnitude of deviation from the median.
Article Snippet: Microarray data RNA was extracted using the RNeasy Kit (Qiagen, Valencia, CA) from 7 BMPC, 7 PPC, 7 PBB, 7 TBC, and the tumor PC purified from 16 patients with MM. cRNA preparation and hybridization to the HuGeneFL GeneChip microarray (Affymetrix, Santa Clara, CA) were done as described ( Tarte , et al 2003 , Zhan , et al 2002 ).
Techniques: Expressing, Purification, Generated